PRODUCTS |  IR VIVO

CHARACTERISTICS          SPECIFICATIONS          APPLICATIONS          PUBLICATIONS          RELATED PRODUCTS         VIDEO


 

Using near and shortwave infrared, IR VIVO benefits from reduced scattering as well as minimal tissue absorption and auto-fluorescence. This allows deeper, clearer imaging than standard optical imaging. IR VIVO takes advantage of the most recent developments in SWIR imaging. It combines Photon etc.’s ultra-low noise InGaAs camera (ZephIR™ 1.7) with novel illumination and powerful analytical software to provide an unprecedented combination of fast, high resolution and deep imaging.

 

NIR-II IMAGING PROPERTIES

  • High spatial resolution

  • High temporal resolution (real-time dynamics)

  • Non-ionizing & non-invasive

  • Good penetration depth (10x greater than traditional visible optical systems) 

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PRODUCT SPECIFICATIONS 

IR VIVO

EMISSION SPECTRAL RANGE  

850 - 1620 nm
Extension available in the visible up to 400 nm

FILTER TYPES   Multispectral: Filter wheel
Hyperspectral: HyperCube™
HYPERCUBE SPECTRAL RESOLUTION  < 4 nm
ILLUMINATION SOURCE  

730, 780, 808 or 810 nm LEDs
Other sources available upon request

ILLUMINATION AREA   15.6 x 12.5 cm field of view for 3 mice model
FIELD OF VIEW   variable from 5 x 4 cm to 15.6 x 12.5 cm
OVERALL INSTRUMENT   DIMENSIONS  

Tabletop
About 75 x 115 x 75 cm

STAGE TEMPERATURE   Up to 40ºC
ANESTHETIC TUBING AND   NOSECONE   3-mice anesthetic gas manifold supplied
COMPUTER   PC with Windows 7-64 bits included
IMAGING SOFTWARE   PHySpec™ software
SPECTRAL DATA FORMAT  HDF5, FITS
SINGLE IMAGE DATA FORMAT  HDF5, CSV, JPG, PNG, TIFF
   
POWER REQUIREMENT   120 VAC / 12 A / 60 Hz
ACQUISITION MODE AVAILABLE   Unfiltered (Broadband)
VIDEO MODE   Megapixel camera for sample visualization

----  CAMERA  ----

TYPE   InGaAs (ZephIR™ 1.7)
FPA  

640 x 512 pixels

PIXEL SIZE   15 µm
QE   < 75%
DYNAMIC RANGE   13/15 bits

APPLICATIONS

The increased penetration depth and contrast of NIR-II imaging combined with its fast acquisition speed and micron-scale spatial resolution enable to visualize simultaneously microvasculature and blood flow through an intact cranial bone. Other biological applications include pharmacokinetics of chemotherapy drugs and lipid quantification in liver and blood circulation. 

PUBLICATIONS


REAL TIME IN VIVO IMAGING IF ICG IN THE NIR-II
with IR VIVO Imaging system

We sought to develop a near infrared II small animal imaging system which could provide real time images and videos of shortwave IR (SWIR) fluorescent signals in vivo at wavelengths over 1000 nm. It was hypothesized that the SWIR wavelengths would give optimal resolution for in vivo optical imaging due to the low tissue autofluorescence, scattering and absorption of light at these wavelengths. The desired preclinical imaging system should enable measurement of heart rate, respiratory rate, hepatic function, hepatobiliary and intestinal function, blood flow and angiography in small animals.

   


INSIGHT FROM THE INDUSTRY - IR VIVO
USING PRE-CLINICAL IMAGING TO DETECT CANCER
By Émilie Beaulieu-Ouellet, Application Scientist in Life Science

In this interview, Émilie Beaulieu Ouellet talks about the recently released and breakthrough imaging system: the IR VIVO™ and its technology. Photon etc.’s IR VIVO™ system is the first and only turnkey hyperspectral preclinical imager optimized for imaging in the second biological window of the near-infrared (NIR-II) / short-wavelength infrared (SWIR) range available on the market to our knowledge. NIR-II imaging will bring an unprecedented combination of fast, high resolution and penetration depth imaging at lower cost and to a broader community than current preclinical imaging techniques. Altogether, it will enable to resolve and track single biomedical targets or processes throughout small animals, thereby opening a new window of possibilities for fundamental and biopharmaceutical research.

RELATED PRODUCTS

ZEPHIR™ 1.7

ZephIR™ 1.7 is an affordable scientific-grade near-infrared InGaAs camera, highly sensitive from 0.8 to 1.7 µm. A four-stage TE cooler, deep-cooling at -80°C, provides unrivalled low-noise levels at an astounding 200 frame-per-second rate.

HYPERCUBE

The HyperCube will transform your microscope, camera or a vast variety of excitation modules into a high resolution spectral imaging system, opening new research perspectives in biological imaging. 

VIDEOS

Photon etc.'s IR VIVO system

Video of IR VIVO imaging experiment showing the biodistribution and pharmacokinetics of ICG in real-time. IR VIVO is able to visualize basic NIR probes at high resolutions allowing to address questions in biology in ways not yet imagined.  From one single injection, it was possible to obtain measurements of heartbeat, respiration and intestinal contraction rates. 

Photon etc.'s Global Imaging Technology

This video shows the conceptual difference between hyperspectral global imaging and raster scan (line-scan, push-  broom). With global imaging, the gain in acquiring 3D data, 2D  spatial and 1D spectral, is important since the only a few monochromatic images are required to cover the complete spectral range where one needs to take the full spectrum for each point or line in the image with other technologies.